Methods applied

Here are some of the methods we apply in our lab.

In vivo and Ex vivo (metabolic) tests: glucose tolerance test, insulin tolerance test, pyruvate tolerance test, glucagon tolerance test, lipid tolerance test, hepatic triacylglycerol exportation, hepatic insulin clearance, glucose-induced insulin secretion, fat glycerol release, rotarod test, open field test, step-down memory test.

Molecular tools: Elisa, Flow cytometry, Western blot, PCR.

Histological tools: immunoperoxidase, immunofluorescence, confocal approaches, TEM, SEM, and tissue and cell morphometries.

Species investigated: Wistar rats, Swiss mice, and humans.